Journal: NPJ Science of Food

Article Title: Umami induces pulmonary epithelial senescence via L-glutamic acid-triggered minority MOMP

doi: 10.1038/s41538-026-00783-x

Figure Lengend Snippet: A Schematic diagram of Mem treatment in high-MSG diet mice. B – D Pulmonary function parameters ( B ) IC, ( C ) Ers, and ( D ) Crs in high-MSG diet mice treated with Mem. E – G Representative H&E-stained lung sections ( E ) and quantitative analysis of lung injury area F , G in Mem-treated mice. H Representative Masson’s trichrome staining showing fibrosis in Mem-treated mice. I , J Representative mIHC images ( I ) and quantitative analysis ( J ) of AQP5 and P21 co-staining in Mem-treated mice. K Representative images of SA-β-gal staining in A549 cells treated with Glu and MK-801. L , M Western blot analysis ( L ) and quantitative analysis ( M ) of P21 protein levels in A549 cells treated with Glu and MK-801. N mRNA expression levels of SASP factors IL-6 and IL-8 in A549 cells treated with Glu and MK-801. All data are presented as mean ± SEM. ns no significance, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. MSG group as determined by one-way ANOVA.

Article Snippet: In specified conditions, cells were co-treated with 10 μM Z-VAD-FMK (MedChemExpress, China), 10 μM CCCP (C2003S, Beyotime, China), or 50 μM MK-801 (T6259, TargetMol, USA).

Techniques: Staining, Western Blot, Expressing